The major interest of our laboratory is the cellular and molecular mechanisms of acute lung injury in lung transplantation.
We are focusing on the ischemia-reperfusion induced lung injury in lung transplantation, especially on the role of different types of inflammation related programmed cell death. Bioinformatics, immunohistochemistry and ELISA are used to determine changes of cell death signals in patient plasma and in ex vivo lung perfusion (EVLP) perfusate. The results are correlated with clinical outcome to select biomarkers for donor lung assessment.
Another major research project is to develop new donor lung preservation solution and EVLP perfusion solution. Cell culture models are used for high throughput screening of components, concentrations and combinations of solution. Selected formulas are tested on animal lung transplantation and EVLP to determine improvement of donor lung function. The selected solution will be used to enable donor lung reconditioning (e.g., gene editing) and to improve clinical lung transplant outcome.