Cellular and Molecular Mechanisms of Neuronal Damage During Stroke and Other Injuries
Research in my lab is centered around the cellular and molecular mechanisms of neuronal damage during stroke and other injuries such as epilepsy and trauma.

Our focus is on calcium-regulated signaling pathways that cause neurons to die when injured. Our main contributions to this field has been the determination that acute damage to neurons is mediated by distinct signaling pathways that are specifically associated with certain types of cell membrane receptors and ion channels.

We are working on elucidating these pathways, with the goal of finding rate-limiting steps that might be amenable to modulation by pharmacological or genetic means. The ultimate goal therefore, is to design rational therapeutic strategies for neuronal injury based on a concrete understanding of the molecular mechanisms that cause it.

Current projects:
  1. We are working on mechanisms of coupling of Ca2+ influx through the NMDA subtype of glutamate receptors to intracellular second messengers that trigger neurotoxic signaling pathways. This project is centered around an understanding and modulation of the molecular organization of synapses, and the treatment of neuronal injury triggered by postsynaptic glutamate receptors.
  2. We are working on mechanisms of anoxic cell damage that are independent of postsynaptic glutamate receptors. Current projects involve the elucidation of Ca2+-dependent intracellular signaling pathways that result in cell death in the absence of excitotoxicity (glutamate-receptor-mediated damage).
  3. Using transgenic mice, we are working on the mechanisms of neurotoxicity triggered by non-NMD (AMPA-type) glutamate receptors in excitotoxicity.
The scope of each project includes studies in tissue culture and in-vivo models. Techniques most commonly used are neurophysiological, including Ca2+ imaging, confocal imaging, electrophysiology, molecular biology, and protein chemistry.
Neurosurgery. 2019 Nov 21;:
van der Zwan A, Vajkoczy P, Amin-Hanjani S, Charbel FT, Welch B, Tymianski M, Kivipelto L, van Thoor S, Chakraborty S, O'Donnell D, Langer DJ
Stroke. 2019 Aug;50(8):2259-2269
Lenck S, Nicholson P, Tymianski R, Hilditch C, Nouet A, Patel K, Krings T, Tymianski M, Radovanovic I, Mendes Pereira V
Stroke. 2019 Jun;50(6):1634-1635
Cordonnier C, Tymianski M
J Neurosurg. 2019 Feb 22;:1-11
Andrade-Barazarte H, Patel K, Turel MK, Doglietto F, Agur A, Gentili F, Tymianski R, Mendes Pereira V, Tymianski M, Radovanovic I
J Neurosci. 2019 Jan 29;:
Gondard E, Teves L, Wang L, McKinnon C, Hamani C, Kalia SK, Carlen PL, Tymianski M, Lozano AM
J Neurosurg. 2018 Jul 27;:1-8
Jin H, Lenck S, Krings T, Agid R, Fang Y, Li Y, Kostynskyy A, Tymianski M, Pereira VM, Radovanovic I
J Clin Neurosci. 2018 Jun 12;:
Rosen C, McKetton L, Russell J, Sam K, Poublanc J, Crawley A, Han JS, Sobczyk O, Duffin J, Mandell DM, Tymianski M, Fisher JA, Mikulis DJ, Venkatraghavan L
World Neurosurg. 2018 Jun 01;:
Lenck S, Schwartz M, Hengwei J, Agid R, Nicholson P, Krings T, Tymianski M, Mendes-Pereira V, Radovanovic I
J Neurosurg Sci. 2018 May 28;:
Hilditch CA, Brinjikji W, Tsang AC, Nicholson P, Kostynskyy A, Tymianski M, Krings T, Radovanovic I, Pereira VM



Professor, Department of Surgery (Neurosurgery), University of Toronto
Professor, Institute of Medical Science, University of Toronto
Cross appointed as Professor, Dept of Physiology, University of Toronto