Princess Margaret Genomics Centre

Genomics core facility offering genomics and validation applications. Dr. Trevor Pugh is the Scientific Director of PMGC.

Overview
  • Certified Agilent service provider and authorized Affymetrix service provider
  • Core facility for Illumina, ION Torrent, Luminex and NanoString Technologies
  • Completed over 1,100 research projects between 2007 and 2014
Services
  • Deep Sequencing: Exome-Seq, ChIP-Seq, Methyl-Seq, RNA-Seq, Targeted Resequencing
  • Data analysis and informatics support for NGS
  • Sequencing library preparation
  • Affymetrix GeneChip (Gene expression, all-exon arrays, SNP arrays)
  • Agilent (Gene expression, arrayCGH, ChIP-on-Chip and methylation, microRNA arrays)
  • Illumina (whole genome and genotyping) BeadChips
  • Luminex multiplex analysis of biomarkers (cytokines, chemokines, signalling factors, etc.)
  • NanoString (Amplification-free quantitative validation of gene expression and CNV studies)
  • Quantitative PCR, Cancer biomarker and CNV studies
  • Gene expression (mRNA and miRNA) services
  • ChIP-on-chip, DNA methylation and epigenetics services
  • Genotyping (CNV and SNP) services
  • Experimental design and bioinformatics consultation
  • Microarray data analysis, informatics and data mining
  • Microarray imaging and quantification

 

Using the Facility
  • Products and services available to all UHN researchers and external academic groups. Some services also available to non-academic organizations.

Usage Arrangements

Sample Preparation
See Protocols: http://www.pmgenomics.ca/ (link is external).

Available Equipment
  • High-throughput Sequencers (Illumina HiSeq 2000 and Ion Torrent Proton)
  • Scanner (Agilent, 2-micron high resolution)
  • Scanner (Affymetrix GeneChip scanner and Illumina iScan system)
  • Luminex (Bio-Rad Bio-Plex reader and bead-based multiplexing technology)
  • NanoString nCounter System (Digital amplification-free gene expression system)
  • Digital PCR (Bio-Rad QX200 Droplet Digital PCR System)
  • Fluorescent Plate Readers (Beckman Coulter Multimode Detector and Tecan Infinite 200 PRO reader)
  • Bioanalyzer (Agilent 2100 Bioanalyzer and 2200 TapeStation)
  • NanoDrop 1000 (NanoDrop)
  • Inkjet array printer (Arrayjet, non-contact inkjet printer for protein/peptide microarrays)
  • Microarray printer (Contact printer using Stealth pins)
Quick Links
Contacts

Neil Winegarden
Manager
416-581-7440
Fax: 416-597-0100
The Princess Margaret Genomics Centre
Princess Margaret Cancer Research Tower
101 College Street, Rm 9-301, Toronto, Ontario, M5G 1L7

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Genomics core facility offering genomics and validation applications. Dr. Trevor Pugh is the Scientific Director of PMGC.

Overview
  • Certified Agilent service provider and authorized Affymetrix service provider
  • Core facility for Illumina, ION Torrent, Luminex and NanoString Technologies
  • Completed over 1,100 research projects between 2007 and 2014
Services
  • Deep Sequencing: Exome-Seq, ChIP-Seq, Methyl-Seq, RNA-Seq, Targeted Resequencing
  • Data analysis and informatics support for NGS
  • Sequencing library preparation
  • Affymetrix GeneChip (Gene expression, all-exon arrays, SNP arrays)
  • Agilent (Gene expression, arrayCGH, ChIP-on-Chip and methylation, microRNA arrays)
  • Illumina (whole genome and genotyping) BeadChips
  • Luminex multiplex analysis of biomarkers (cytokines, chemokines, signalling factors, etc.)
  • NanoString (Amplification-free quantitative validation of gene expression and CNV studies)
  • Quantitative PCR, Cancer biomarker and CNV studies
  • Gene expression (mRNA and miRNA) services
  • ChIP-on-chip, DNA methylation and epigenetics services
  • Genotyping (CNV and SNP) services
  • Experimental design and bioinformatics consultation
  • Microarray data analysis, informatics and data mining
  • Microarray imaging and quantification

 

Using the Facility
  • Products and services available to all UHN researchers and external academic groups. Some services also available to non-academic organizations.

Usage Arrangements

Sample Preparation
See Protocols: http://www.pmgenomics.ca/ (link is external).

Available Equipment
  • High-throughput Sequencers (Illumina HiSeq 2000 and Ion Torrent Proton)
  • Scanner (Agilent, 2-micron high resolution)
  • Scanner (Affymetrix GeneChip scanner and Illumina iScan system)
  • Luminex (Bio-Rad Bio-Plex reader and bead-based multiplexing technology)
  • NanoString nCounter System (Digital amplification-free gene expression system)
  • Digital PCR (Bio-Rad QX200 Droplet Digital PCR System)
  • Fluorescent Plate Readers (Beckman Coulter Multimode Detector and Tecan Infinite 200 PRO reader)
  • Bioanalyzer (Agilent 2100 Bioanalyzer and 2200 TapeStation)
  • NanoDrop 1000 (NanoDrop)
  • Inkjet array printer (Arrayjet, non-contact inkjet printer for protein/peptide microarrays)
  • Microarray printer (Contact printer using Stealth pins)
Quick Links
Contacts

Neil Winegarden
Manager
416-581-7440
Fax: 416-597-0100
The Princess Margaret Genomics Centre
Princess Margaret Cancer Research Tower
101 College Street, Rm 9-301, Toronto, Ontario, M5G 1L7