The Real-time PCR facility is a fee-for-service facility with sites at Princess Margaret (PM) Cancer Centre, Krembil Discovery Tower (KDT), and Princess Margaret Cancer Research Tower (PMCRT, formerly TMDT). The facilities are managed by Research Laboratory Services.
Anyone would like to book the RT-PCR machines, please contact Wynne Yiu
site | system |
---|---|
PM | Applied Biosystems Sequence Detection Systems 7900HT
Thermo Quant Studio 6 Pro RT-PCR System
Roche Light Cycler 480 II
|
KDT | Thermo QuantStudio 5 RT-PCR System
|
PMCRT | Applied Biosystems Sequence Detection System 7900HT
Roche Light Cycler 480 II
Thermo QuanStudio 6 Pro RT-PCR System
|
- Users can book the instrument using the QReserve. Please refer to QReserve Sign Up Guide.
- RT-PCR is considered as specialized equipment. Before you can reserve the equipment, please refer to QReserve-Specialized Equipment Guide.
- Each time slot is 2.5 hours, which allows 2 runs and brief data analysis
- Minimum 24 hours notification is required for any cancelations
Training
See the following documents before starting your RT-PCR experiments
- ABI SDS7900
- Roche LightCycler 480
- Thermo QuantStudio 6 Pro
Techinical Support
- For technical support, contact Raymond Huynh (at PM), Frank Vidic (at KDT), Aaliya Tamachi (at PMCRT).
Account Registration (All sites):
Kirstie Fraser, Administative Assistant
Please Contact Your Site Facilities Coordinator for Support:
- Princess Margaret- Raymond Huynh (416-684-4620)
- PMCRT- Aaliya Tamachi (437-218-3233)
- KDT- Frank Vidic (416-603-6462)