Access will require clientele registration, and an introduction to the facility policies, and instruments. This will ensure that the UHN biosafety guidelines are met. 

 

Overview

Toronto Western Hospital
Krembil Discovery Tower.
60 Leonard Street.
5th floor, room #443.

 

The Facility is operated as a Fee for service Facility.

We are open to all UHN Investigators and External Clientele.

Access will require clientele registration, and an introduction to the facility policies, and instruments. This will ensure that the UHN biosafety guidelines are met.  New personnel will require training for the instruments they wish to use, and once training has been completed they will be provided security access to the facility, and a login password to access our online booking system.  Please see Getting Started-Internal / Getting Started-External.

Facility staff are available between the hours of 10:00 am and 5:00 pm Monday-Friday.  Staff will try and accommodate clients outside of these hours where possible, however additional charges will be applied.

 Access for Internal users is unlimited.

Contact Edwin.Speck@uhnresearch.ca

Available Instruments

There are three cytometers.  Two analyzers and one cell sorter.

Please note we do have a assortment of extra mirrors and filters which can be exchanged, thereby allowing the detection of other fluorochromes not listed.

The Analyzers

 

Cell Sorter

KDT-UHN Flow Cytometry Guideline

All users must read the KDT Flow Cytometry Facility policies below, checking off all points after   they have read them, submitting the completed form to the flow facility.

Guidelines for Facilities Use

☐ Only trained users, that have completed the General Research Safety Training, Wet Lab Safety Training, and whose PI possess a valid UHN Research Biosafety Certificate, which must cover the work being conducted in the FCF (Flow Cytometry Facility) are allowed to use these facilities.  Please provide documentation.  See Getting Started - Internal / Getting Started - External

☐ Initial training must be completed by FCF staff.  Other trained users may assist in post training but, must remain with the trainee at all times.  An assessment of the user will be determined before they can independently use this facility.

☐ Access is restricted to FCF staff and registered users of the facility who have been issued a security access card.  This security access card may not be used by or transferred to anyone other than the person to whom it is issued.

☐ Personal login accounts on analytical instruments must only be used by the person to whom the account belongs.

Using the Facility

☐ You are required to bring your own personal protective equipment such as lab coats if you are located on 5KDT.  If you reside on another floor you may bring a clean Lab coat or one will be provided for you

Samples

Ensure your samples are clump free, and filtered prior to analysis.

After each use

☐ Clean the analyzer according to the SOP associated with each instrument.

☐Discard the bleach treated waste (allow 30 minutes for decontamination) from the fluid container down the sink, and add bleach to the empty container.

☐ Replenish the sheath fluid container.

☐ Remove tubes, tips and any other materials you have brought into the facility as biohazardous waste bins are not provided.

☐ Clean the work area as discussed during training.

  ☐ If you encounter any problems: clogs, error messages please report this to a FCF staff member

  ☐ The transportation of all primary samples (tubes) must be sealed (capped, and no open tubes in racks).  In addition, all sealed primary containers must be transported to the facility in a secondary, sealed, leak-proof container.  The leak-proof secondary container (e.g. ‘Tupperware’-type containers with a tight fitting lid) must be compliant with the Transportation of Dangerous Goods Act 

  ☐ I have read and understand all the statements, policies and procedures above and presented to me, and will abide by the KDT-UHN Flow Facility and UHN Policies and Procedures.

Name of Employee/Student(print):     
Employee/Student signature:  
Date:
 Name of Principal Investigator:  

 Download and Print a Copy of the Guidelines

Once completed, Sign and Forward to Edwin.Speck@uhnresearch.ca

 

 

Contacts

Edwin Speck
Flow Facility
Krembil Research Institute
Krembil Discovery Tower, 5KD443
60 Leonard Avenue Toronto, Ontario, M5T 2S8
Email: Edwin.Speck@uhnresearch.ca
Phone: 416-684-5742

Access will require clientele registration, and an introduction to the facility policies, and instruments. This will ensure that the UHN biosafety guidelines are met. 

 

Overview

Toronto Western Hospital
Krembil Discovery Tower.
60 Leonard Street.
5th floor, room #443.

 

The Facility is operated as a Fee for service Facility.

We are open to all UHN Investigators and External Clientele.

Access will require clientele registration, and an introduction to the facility policies, and instruments. This will ensure that the UHN biosafety guidelines are met.  New personnel will require training for the instruments they wish to use, and once training has been completed they will be provided security access to the facility, and a login password to access our online booking system.  Please see Getting Started-Internal / Getting Started-External.

Facility staff are available between the hours of 10:00 am and 5:00 pm Monday-Friday.  Staff will try and accommodate clients outside of these hours where possible, however additional charges will be applied.

 Access for Internal users is unlimited.

Contact Edwin.Speck@uhnresearch.ca

Available Instruments

There are three cytometers.  Two analyzers and one cell sorter.

Please note we do have a assortment of extra mirrors and filters which can be exchanged, thereby allowing the detection of other fluorochromes not listed.

The Analyzers

 

Cell Sorter

KDT-UHN Flow Cytometry Guideline

All users must read the KDT Flow Cytometry Facility policies below, checking off all points after   they have read them, submitting the completed form to the flow facility.

Guidelines for Facilities Use

☐ Only trained users, that have completed the General Research Safety Training, Wet Lab Safety Training, and whose PI possess a valid UHN Research Biosafety Certificate, which must cover the work being conducted in the FCF (Flow Cytometry Facility) are allowed to use these facilities.  Please provide documentation.  See Getting Started - Internal / Getting Started - External

☐ Initial training must be completed by FCF staff.  Other trained users may assist in post training but, must remain with the trainee at all times.  An assessment of the user will be determined before they can independently use this facility.

☐ Access is restricted to FCF staff and registered users of the facility who have been issued a security access card.  This security access card may not be used by or transferred to anyone other than the person to whom it is issued.

☐ Personal login accounts on analytical instruments must only be used by the person to whom the account belongs.

Using the Facility

☐ You are required to bring your own personal protective equipment such as lab coats if you are located on 5KDT.  If you reside on another floor you may bring a clean Lab coat or one will be provided for you

Samples

Ensure your samples are clump free, and filtered prior to analysis.

After each use

☐ Clean the analyzer according to the SOP associated with each instrument.

☐Discard the bleach treated waste (allow 30 minutes for decontamination) from the fluid container down the sink, and add bleach to the empty container.

☐ Replenish the sheath fluid container.

☐ Remove tubes, tips and any other materials you have brought into the facility as biohazardous waste bins are not provided.

☐ Clean the work area as discussed during training.

  ☐ If you encounter any problems: clogs, error messages please report this to a FCF staff member

  ☐ The transportation of all primary samples (tubes) must be sealed (capped, and no open tubes in racks).  In addition, all sealed primary containers must be transported to the facility in a secondary, sealed, leak-proof container.  The leak-proof secondary container (e.g. ‘Tupperware’-type containers with a tight fitting lid) must be compliant with the Transportation of Dangerous Goods Act 

  ☐ I have read and understand all the statements, policies and procedures above and presented to me, and will abide by the KDT-UHN Flow Facility and UHN Policies and Procedures.

Name of Employee/Student(print):     
Employee/Student signature:  
Date:
 Name of Principal Investigator:  

 Download and Print a Copy of the Guidelines

Once completed, Sign and Forward to Edwin.Speck@uhnresearch.ca

 

 

Contacts

Edwin Speck
Flow Facility
Krembil Research Institute
Krembil Discovery Tower, 5KD443
60 Leonard Avenue Toronto, Ontario, M5T 2S8
Email: Edwin.Speck@uhnresearch.ca
Phone: 416-684-5742