Mechanisms of neural synchrony and entrainment (epilepsy), and neurodegenerative processes
We have several projects on cellular mechanisms of epilepsy, particularly the synchronizing role of electrotonic coupling via gap junctions. Molecular biological and cellular electrophysiological recording techniques are being used to measure the upregulation of gap junctional function in several in vitro seizure models, including the use of the intact mouse hippocampus preparation. Also a project on the pathogenesis of hypoglycemic seizures is in progress.
In collaboration with Drs. Berj Bardakjian and Frances Skinner, the linear and nonlinear electrical and network properties of central mammalian neurons in physiological and pathophysiological conditions (e.g., epilepsy) are being described by neural modelling techniques. We are developing nonlinear techniques for the identification different brain states including those associated with anesthesia and epilepsy.
In models of stroke and Alzheimer's disease, calcium homeostasis and free radical production are under investigation, focusing on the role of degenerating mitochondrial function in presynaptic terminals. Fluorescence and confocal microscopic imaging of intracellular calcium and mitochondrial function coupled with whole cell and field electrophysiological recordings are being used.
In collaboration with Drs. Bhushan Kapur, James Reynolds and James Brien, we are examining the role of formic acid in the causation of the brain damage in the fetal alcohol spectrum disorder and its rescue by folate.
