Dr. Sugita’s primary research interest is to understand the molecular mechanisms underpinning exocytosis using neuronal and immune cells with particular emphasis on the roles of Munc18, syntaxin, Munc13, CAPS1 and V-ATPase. To this end, he uses PC12 cells and RBL-2H3 cells as cellular models for neuroendocrince cells and mast cells, respectively, and uses C. elegans as an in vivo model. Moreover, conditional knockout mice are being generated to analyze the function of these proteins in vitro and in vivo. These models will be used to show how Munc18-1 contributes to neuroprotection, thereby explaning why its loss in neurons leads to severe neurodegeneration.
J Immunol. 2018 Jun 08;:
Crucial Role of Postsynaptic Syntaxin 4 in Mediating Basal Neurotransmission and Synaptic Plasticity in Hippocampal CA1 Neurons.
Cell Rep. 2018 Jun 05;23(10):2955-2966
Biochem Soc Trans. 2018 Feb 22;:
Continuous Monitoring via Tethered Electroencephalography of Spontaneous Recurrent Seizures in Mice.
Front Behav Neurosci. 2017;11:172
UNC-18 and Tomosyn antagonistically control synaptic vesicle priming downstream of UNC-13 in C. elegans.
J Neurosci. 2017 Aug 08;:
Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion.
Elife. 2017 May 06;6:
Munc18b Increases Insulin Granule Fusion, Restoring Deficient Insulin Secretion in Type-2 Diabetes Human and Goto-Kakizaki Rat Islets with Improvement in Glucose Homeostasis.
EBioMedicine. 2017 Jan 22;:
Curr Biol. 2017 Jan 09;:
ϒ-secretase and LARG mediate distinct RGMa activities to control appropriate layer targeting within the optic tectum.
Cell Death Differ. 2016 Mar;23(3):442-53
Anesthesiology. 2016 Oct;125(4):822-3
Senior Scientist, Krembil Research Institute (Krembil)
Professor, Department of Physiology, University of Toronto