Philippe P Monnier

Philippe P Monnier, MSc, MBA, PhD

Our main interests are the mechanisms that govern axonal outgrowth and cell migration in the central nervous system during development.

a) We have several projects aimed at the identification of membrane components that guide neurons to their target cells. Our main focus is the characterization of matrix  and membrane proteins involved in retina ganglion cell axon outgrowth. These studies use culture of retina explants, as well as other strategies in which protein expression is manipulated in the eye (using electroporation).

b) We developed several in vitro models (explant culture, organotypic cultures) to analyze the role of membrane and extracellular matrix proteins on cell migration in the central nervous system (CNS). We are concentrating our efforts on understanding the role of the newly identified membrane protein RGM on progenitor cell migration in the forebrain and the retina.

c) We recently purified the new matrix protein T61. In vitro experiments using blocking antibodies demonstrate that T61 is part of the substrate that allows axonal growth in the retina. Our goal is to use the purified protein to get peptide data and clone the full length protein. Using recombinant T61 we will confirm its biological function as part of the environment that allows axonal growth.
Sci Rep. 2017 Sep 05;7(1):10529
Mothe AJ, Tassew NG, Shabanzadeh AP, Penheiro R, Vigouroux RJ, Huang L, Grinnell C, Cui YF, Fung E, Monnier PP, Mueller BK, Tator CH
J Neurosci. 2017 Aug 08;:
Park S, Bin NR, Yu B, Wong R, Sitarska E, Sugita K, Ma K, Xu J, Tien CW, Algouneh A, Turlova E, Wang S, Siriya P, Shahid W, Kalia L, Feng ZP, Monnier PP, Sun HS, Zhen M, Gao S, Rizo J, Sugita S
Cell Rep. 2017 Jul 05;20(1):99-111
Tassew NG, Charish J, Shabanzadeh AP, Luga V, Harada H, Farhani N, D'Onofrio P, Choi B, Ellabban A, Nickerson PEB, Wallace VA, Koeberle PD, Wrana JL, Monnier PP
Trends Cell Biol. 2016 Dec 19;:
Siebold C, Yamashita T, Monnier PP, Mueller BK, Pasterkamp RJ
Elife. 2016 Nov 08;5:
Bassett EA, Tokarew N, Allemano EA, Mazerolle C, Morin K, Mears AJ, McNeill B, Ringuette R, Campbell C, Smiley S, Pokrajac NT, Dubuc AM, Ramaswamy V, Northcott PA, Remke M, Monnier PP, Potter D, Paes K, Kirkpatrick LL, Coker KJ, Rice DS, Perez-Iratxeta...
Mol Biol Cell. 2015 Dec 23;
Park S, Bin NR, Michael Rajah M, Kim B, Chou TC, Kang SA, Sugita K, Parsaud L, Smith M, Monnier PP, Ikura M, Zhen M, Sugita S
Cell Death Dis. 2015;6:e1967
Shabanzadeh AP, D'Onofrio PM, Monnier PP, Koeberle PD
Cell Death Differ. 2015 Aug 21;
Banerjee P, Harada H, Tassew NG, Charish J, Goldschneider D, Wallace VA, Sugita S, Mehlen P, Monnier PP
Cell Death Dis. 2015;6:e1744
Shabanzadeh AP, Tassew NG, Szydlowska K, Tymianski M, Banerjee P, Vigouroux RJ, Eubanks JH, Huang L, Geraerts M, Koeberle PD, Mueller BK, Monnier PP
Cell Rep. 2015 Mar 17;
Demicheva E, Cui YF, Bardwell P, Barghorn S, Kron M, Meyer AH, Schmidt M, Gerlach B, Leddy M, Barlow E, O'Connor E, Choi CH, Huang L, Veldman GM, Rus H, Shabanzadeh AP, Tassew NG, Monnier PP, Müller T, Calabresi PA, Schoemaker H, Mueller BK

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Associate Professor, Department of Physiology, University of Toronto
Associate Professor, Department of Ophthalmology, University of Toronto