Philippe P Monnier, MSc, MBA, PhD

Our main interests are the mechanisms that govern axonal outgrowth and cell migration in the central nervous system during development.

a) We have several projects aimed at the identification of membrane components that guide neurons to their target cells. Our main focus is the characterization of matrix  and membrane proteins involved in retina ganglion cell axon outgrowth. These studies use culture of retina explants, as well as other strategies in which protein expression is manipulated in the eye (using electroporation).

b) We developed several in vitro models (explant culture, organotypic cultures) to analyze the role of membrane and extracellular matrix proteins on cell migration in the central nervous system (CNS). We are concentrating our efforts on understanding the role of the newly identified membrane protein RGM on progenitor cell migration in the forebrain and the retina.

c) We recently purified the new matrix protein T61. In vitro experiments using blocking antibodies demonstrate that T61 is part of the substrate that allows axonal growth in the retina. Our goal is to use the purified protein to get peptide data and clone the full length protein. Using recombinant T61 we will confirm its biological function as part of the environment that allows axonal growth.
Nat Chem Biol. 2019 Aug 26;:
Harada H, Farhani N, Wang XF, Sugita S, Charish J, Attisano L, Moran M, Cloutier JF, Reber M, Bremner R, Monnier PP
Cell Death Dis. 2019 Jul 29;10(8):567
Shabanzadeh AP, D'Onofrio PM, Magharious M, Choi KAB, Monnier PP, Koeberle PD
Biomed Opt Express. 2018 Nov 01;9(11):5615-5634
Ringuette D, Nauenberg J, Monnier PP, Carlen PL, Levi O
Mol Neurobiol. 2018 Aug 17;:
Deurloo MHS, Turlova E, Chen WL, Lin YW, Tam E, Tassew NG, Wu M, Huang YC, Crawley JN, Monnier PP, Groffen AJA, Sun HS, Osborne LR, Feng ZP
J Immunol. 2018 Jun 08;:
Bin NR, Ma K, Tien CW, Wang S, Zhu D, Park S, Turlova E, Sugita K, Shirakawa R, van der Sluijs P, Horiuchi H, Sun HS, Monnier PP, Gaisano HY, Sugita S
Cell Rep. 2018 Jun 05;23(10):2955-2966
Bin NR, Ma K, Harada H, Tien CW, Bergin F, Sugita K, Luyben TT, Narimatsu M, Jia Z, Wrana JL, Monnier PP, Zhang L, Okamoto K, Sugita S
Cell Death Differ. 2018 Feb 02;:
Zhang R, Wu Y, Xie F, Zhong Y, Wang Y, Xu M, Feng J, Charish J, Monnier PP, Qin X
J Stroke Cerebrovasc Dis. 2017 Nov 29;:
Shabanzadeh AP, D'Onofrio PM, Monnier PP, Koeberle PD
Sci Rep. 2017 Sep 05;7(1):10529
Mothe AJ, Tassew NG, Shabanzadeh AP, Penheiro R, Vigouroux RJ, Huang L, Grinnell C, Cui YF, Fung E, Monnier PP, Mueller BK, Tator CH
J Neurosci. 2017 Aug 08;:
Park S, Bin NR, Yu B, Wong R, Sitarska E, Sugita K, Ma K, Xu J, Tien CW, Algouneh A, Turlova E, Wang S, Siriya P, Shahid W, Kalia L, Feng ZP, Monnier PP, Sun HS, Zhen M, Gao S, Rizo J, Sugita S



Associate Professor, Department of Physiology, University of Toronto
Associate Professor, Department of Ophthalmology, University of Toronto