• Certified Agilent service provider and authorized Affymetrix service provider
• Core facility for Illumina, ION Torrent, Luminex and NanoString Technologies
• Completed over 1,100 research projects between 2007 and 2014

• Deep Sequencing: Exome-Seq, ChIP-Seq, Methyl-Seq, RNA-Seq, Targeted Resequencing
• Data analysis and informatics support for NGS
• Sequencing library preparation
• Affymetrix GeneChip (Gene expression, all-exon arrays, SNP arrays)
• Agilent (Gene expression, arrayCGH, ChIP-on-Chip and methylation, microRNA arrays)
• Illumina (whole genome and genotyping) BeadChips
• Luminex multiplex analysis of biomarkers (cytokines, chemokines, signalling factors, etc.)
• NanoString (Amplification-free quantitative validation of gene expression and CNV studies)
• Quantitative PCR, Cancer biomarker and CNV studies
• Gene expression (mRNA and miRNA) services
• ChIP-on-chip, DNA methylation and epigenetics services
• Genotyping (CNV and SNP) services
• Experimental design and bioinformatics consultation
• Microarray data analysis, informatics and data mining
• Microarray imaging and quantification

• Products and services available to all UHN researchers and external academic groups. Some services also available to non-academic organizations.

• Fee for service
• Collaborations in certain cases
• For additional pricing information: https://www.pmgenomics.ca/pmgenomics

Sample Preparation
See Protocols: http://www.pmgenomics.ca/ (link is external).

• High-throughput Sequencers (Illumina HiSeq 2000 and Ion Torrent Proton)
• Scanner (Agilent, 2-micron high resolution)
• Scanner (Affymetrix GeneChip scanner and Illumina iScan system)
• Luminex (Bio-Rad Bio-Plex reader and bead-based multiplexing technology)
• NanoString nCounter System (Digital amplification-free gene expression system)
• Digital PCR (Bio-Rad QX200 Droplet Digital PCR System)
• Fluorescent Plate Readers (Beckman Coulter Multimode Detector and Tecan Infinite 200 PRO reader)
• Bioanalyzer (Agilent 2100 Bioanalyzer and 2200 TapeStation)
• NanoDrop 1000 (NanoDrop)
• Inkjet array printer (Arrayjet, non-contact inkjet printer for protein/peptide microarrays)
• Microarray printer (Contact printer using Stealth pins)

Troy Ketela, PhD
Staff Scientist II, Princess Margaret Cancer Centre
Head of Operations, Princess Margaret Genomics Centre
9-805 PMCRT, 101 College St.
Toronto, ON M5G 1L7
416-634-8816