Genomics core facility offering genomics and validation applications. Dr. Trevor Pugh is the Scientific Director of PMGC.
Overview
- Certified Agilent service provider and authorized Affymetrix service provider
- Core facility for Illumina, ION Torrent, Luminex and NanoString Technologies
- Completed over 1,100 research projects between 2007 and 2014
Services
- Deep Sequencing: Exome-Seq, ChIP-Seq, Methyl-Seq, RNA-Seq, Targeted Resequencing
- Data analysis and informatics support for NGS
- Sequencing library preparation
- Affymetrix GeneChip (Gene expression, all-exon arrays, SNP arrays)
- Agilent (Gene expression, arrayCGH, ChIP-on-Chip and methylation, microRNA arrays)
- Illumina (whole genome and genotyping) BeadChips
- Luminex multiplex analysis of biomarkers (cytokines, chemokines, signalling factors, etc.)
- NanoString (Amplification-free quantitative validation of gene expression and CNV studies)
- Quantitative PCR, Cancer biomarker and CNV studies
- Gene expression (mRNA and miRNA) services
- ChIP-on-chip, DNA methylation and epigenetics services
- Genotyping (CNV and SNP) services
- Experimental design and bioinformatics consultation
- Microarray data analysis, informatics and data mining
- Microarray imaging and quantification
Using the Facility
- Products and services available to all UHN researchers and external academic groups. Some services also available to non-academic organizations.
Usage Arrangements
- Fee for service
- Collaborations in certain cases
- For additional pricing information: https://www.pmgenomics.ca/pmgenomics
Sample Preparation
See Protocols: http://www.pmgenomics.ca/ (link is external).
Available Equipment
- High-throughput Sequencers (Illumina HiSeq 2000 and Ion Torrent Proton)
- Scanner (Agilent, 2-micron high resolution)
- Scanner (Affymetrix GeneChip scanner and Illumina iScan system)
- Luminex (Bio-Rad Bio-Plex reader and bead-based multiplexing technology)
- NanoString nCounter System (Digital amplification-free gene expression system)
- Digital PCR (Bio-Rad QX200 Droplet Digital PCR System)
- Fluorescent Plate Readers (Beckman Coulter Multimode Detector and Tecan Infinite 200 PRO reader)
- Bioanalyzer (Agilent 2100 Bioanalyzer and 2200 TapeStation)
- NanoDrop 1000 (NanoDrop)
- Inkjet array printer (Arrayjet, non-contact inkjet printer for protein/peptide microarrays)
- Microarray printer (Contact printer using Stealth pins)
Contacts
Troy Ketela, PhD
Staff Scientist II, Princess Margaret Cancer Centre
Head of Operations, Princess Margaret Genomics Centre
9-805 PMCRT, 101 College St.
Toronto, ON M5G 1L7
416-634-8816
Comments
Add new comment