Flow Cytometry Facility at PM/CFIBCR

Equipment and expertise necessary for analyses and cell sorting using flow cytometry. Located in the Princess Margaret Cancer Centre, Rm 8-606, open 24 hours (access card required). Facilities available for UHN and non-UHN users.
 
 

Available Equipment
  • Analyzers
    BD Biosciences FACSCanto
    • 2-laser excitation: 488/ 635 nm
    • 6 fluorescent parameters: (4 blue/ 2 red)
    BD Biosciences FACSCanto II
    • 3-laser excitation: 405/ 488/ 635 nm
    • 8  fluorescent parameters: (2 violet/ 4 blue/ 2 red)
    BD Biosciences FACSCanto II High Throughput System (HTS)
    • 3-laser excitation: 405/ 488/ 635 nm
    • 8 fluorescent parameters: (2 violet/ 4 blue/ 2 red)
    • multi-well plate sampler for high throughput screening (96 and 384 formats)
    BD Biosciences Fortessa
    • 5-laser excitation: 355/ 405/ 488/ 561/ 640 nm
    • 17 fluorescent parameters: (2 UV/  5 violet/ 2 blue / 5 green / 3 red)
    • Interchangeable filter sets available upon request.

    BD Biosciences Fortessa 2

    • 5-laser excitation: 355/ 405/ 488/ 561/ 640 nm
    • 18 fluorescent parameters: (3 UV/  6 violet/ 2 blue / 4 green / 3 red)
    • Interchangeable filter sets available upon request.
    BD Biosciences Fortessa X20-High Throughput System (HTS)
    • 4-laser excitation: 405/ 488 / 532/ 640 nm
    • 13 fluorescent parameters: (3 violet/ 2 blue / 5 green / 3 red)  
    • Interchangeable filter sets available upon request.
    • multi-well plate sampler for high throughput screening (96 and 384 formats)
  • Cell Sorters
    Beckman Coulter Inc., MoFlo XDP (modulator flow cytometer)
    • 5-laser excitation:  355/ 405/ 488/ 561/ 642 nm
    • 14 fluorescent parameters
    • High speed 4-way sorting
    • Automated cloning
    BD Biosciences FACSAriaIIu
    • 3-laser excitation: 355/ 488/ 635 nm
    • 10 fluorescent parameters: (2 UV/  5 blue/ 3 red)
    • High speed 4-way sorting 
    • Automated cloning (96/ 384-well and custom formats)
    Beckman Coulter Inc. MoFlo Astrios EQ High Speed Cell Sorter
    • 4-laser excitation: 405/ 488/ 561/ 642 nm
    • 17 fluorescent parameters: 
    • High speed 6-way sorting 
    • Automated cloning (96/ 384-well and custom formats)
Services
  • Full service analysis available (for more info, contact FCF staff)
  • Mandatory Training provided for all users
  • Please call 416-946-4501 ext. 5237 or email FCF Staff at PMflow@uhnresearch.ca to make an appointment. Established users can book time through the calcium calendar. NOTE: Login accounts may not be used by anyone other than the person to whom the account was issued.
Using the Facility
  • New users: please contact our operators (see “Contacts” below).
  • Established users can book time by clicking on the following link: Online booking calendar. NOTE: Login accounts may not be used by anyone other than the person to whom the account was issued.

Training
All users must be trained by FCF staff before gaining permission to use the facility.
 
Usage Arrangements
Sorters are operated exclusively by FCF staff. All new sort clients are strongly encouraged to consult carefully with the sort operator before arriving. FCF staff can provide advice on how to prepare the samples and can determine in advance the appropriate sorter setup.
FACSCaliburs $20/hour (unassisted); $75/hour (assisted)
FACSCantos $30/hour (unassisted); $75/hour (assisted)
Fortessa/FortessaX20/LSRII $40/hour (unassisted); $75/hour (assisted)
Training $75/hour
Cell sorting $75/hour (assisted)




 
Sample Preparation
Please contact our operators for information on sample preparation.
Quick Links
Contacts
Manager:
Arda Shahinian     ashahini@uhnresearch.ca
416-946-2237

Operators:
Group mailbox: PMflow@uhnresearch.ca
telephone number: (416)-946-4501 (ext 5237)

Aleks Spurmanis     aspurman@uhnresearch.ca
Frances Tong          ftong@uhnresearch.ca
Betty Fan                 xuelingf@uhnresearch.ca

Street Address:
610 University Ave., Rm 8-605
Princess Margaret Cancer Centre
Toronto, ON M5G 2M9
 

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Equipment and expertise necessary for analyses and cell sorting using flow cytometry. Located in the Princess Margaret Cancer Centre, Rm 8-606, open 24 hours (access card required). Facilities available for UHN and non-UHN users.
 
 

Available Equipment
  • Analyzers
    BD Biosciences FACSCanto
    • 2-laser excitation: 488/ 635 nm
    • 6 fluorescent parameters: (4 blue/ 2 red)
    BD Biosciences FACSCanto II
    • 3-laser excitation: 405/ 488/ 635 nm
    • 8  fluorescent parameters: (2 violet/ 4 blue/ 2 red)
    BD Biosciences FACSCanto II High Throughput System (HTS)
    • 3-laser excitation: 405/ 488/ 635 nm
    • 8 fluorescent parameters: (2 violet/ 4 blue/ 2 red)
    • multi-well plate sampler for high throughput screening (96 and 384 formats)
    BD Biosciences Fortessa
    • 5-laser excitation: 355/ 405/ 488/ 561/ 640 nm
    • 17 fluorescent parameters: (2 UV/  5 violet/ 2 blue / 5 green / 3 red)
    • Interchangeable filter sets available upon request.

    BD Biosciences Fortessa 2

    • 5-laser excitation: 355/ 405/ 488/ 561/ 640 nm
    • 18 fluorescent parameters: (3 UV/  6 violet/ 2 blue / 4 green / 3 red)
    • Interchangeable filter sets available upon request.
    BD Biosciences Fortessa X20-High Throughput System (HTS)
    • 4-laser excitation: 405/ 488 / 532/ 640 nm
    • 13 fluorescent parameters: (3 violet/ 2 blue / 5 green / 3 red)  
    • Interchangeable filter sets available upon request.
    • multi-well plate sampler for high throughput screening (96 and 384 formats)
  • Cell Sorters
    Beckman Coulter Inc., MoFlo XDP (modulator flow cytometer)
    • 5-laser excitation:  355/ 405/ 488/ 561/ 642 nm
    • 14 fluorescent parameters
    • High speed 4-way sorting
    • Automated cloning
    BD Biosciences FACSAriaIIu
    • 3-laser excitation: 355/ 488/ 635 nm
    • 10 fluorescent parameters: (2 UV/  5 blue/ 3 red)
    • High speed 4-way sorting 
    • Automated cloning (96/ 384-well and custom formats)
    Beckman Coulter Inc. MoFlo Astrios EQ High Speed Cell Sorter
    • 4-laser excitation: 405/ 488/ 561/ 642 nm
    • 17 fluorescent parameters: 
    • High speed 6-way sorting 
    • Automated cloning (96/ 384-well and custom formats)
Services
  • Full service analysis available (for more info, contact FCF staff)
  • Mandatory Training provided for all users
  • Please call 416-946-4501 ext. 5237 or email FCF Staff at PMflow@uhnresearch.ca to make an appointment. Established users can book time through the calcium calendar. NOTE: Login accounts may not be used by anyone other than the person to whom the account was issued.
Using the Facility
  • New users: please contact our operators (see “Contacts” below).
  • Established users can book time by clicking on the following link: Online booking calendar. NOTE: Login accounts may not be used by anyone other than the person to whom the account was issued.

Training
All users must be trained by FCF staff before gaining permission to use the facility.
 
Usage Arrangements
Sorters are operated exclusively by FCF staff. All new sort clients are strongly encouraged to consult carefully with the sort operator before arriving. FCF staff can provide advice on how to prepare the samples and can determine in advance the appropriate sorter setup.
FACSCaliburs $20/hour (unassisted); $75/hour (assisted)
FACSCantos $30/hour (unassisted); $75/hour (assisted)
Fortessa/FortessaX20/LSRII $40/hour (unassisted); $75/hour (assisted)
Training $75/hour
Cell sorting $75/hour (assisted)




 
Sample Preparation
Please contact our operators for information on sample preparation.
Quick Links
Contacts
Manager:
Arda Shahinian     ashahini@uhnresearch.ca
416-946-2237

Operators:
Group mailbox: PMflow@uhnresearch.ca
telephone number: (416)-946-4501 (ext 5237)

Aleks Spurmanis     aspurman@uhnresearch.ca
Frances Tong          ftong@uhnresearch.ca
Betty Fan                 xuelingf@uhnresearch.ca

Street Address:
610 University Ave., Rm 8-605
Princess Margaret Cancer Centre
Toronto, ON M5G 2M9