Advanced Optical Microscopy Facility (AOMF)

A multi-site facility (PMCRT, TGH and the PM) providing optical microscopy technologies for the observation of fixed cells, live cells, and tissues. The facility is led by Drs. Brian Wilson and Ming-Sound Tsao.
 

Services
 
  • Free consultations to determine which microscopes best suit your application
  • Comprehensive training and support on our various instruments
  • Full-service image capture and analysis (a great way to get started quickly)
  • High-resolution whole-slide scanning service for histology/pathology slides
  • Practical courses on microscopy, including one-on-one hands-on sessions
    (eg: Fundamentals of Microscopy) and larger workshops tailored to your group's applications
  • Software and training for image processing and quantification
Available Equipment
  • PMCRT (15th floor, Room 15-305)
    • Aperio Scanscope XT whole-slide scanner: digitizes entire stained histology slides at incredible resolution
    • New Zeiss LSM700 confocal microscope: optimized for DAPI/FITC/Cy3/Cy5 or equivalent
    • Zeiss LSM710 Two-Photon microscope (and fully-loaded confocal): upright microscope stand optimized for intravital imaging of mice and Second Harmonic Generation (SHG) microscopy
    • Zeiss AxioObserver: inverted microscope with incubator for time lapse live-cell imaging
    • Olympus BX50 motorized upright fluorescence microscope: for multi-colour fast tiling of whole slides
    • Zeiss AxioImager Z1 upright fluorescence microscope: has both monochrome and colour cameras
    • Renishaw Raman microscope: with inverted microscope stand for live-celll imaging, 3 lasers, and highspeed Raman mapping mode
    • CompuCyte iCys laser scanning cytometer: for multi-parameter analysis of cells and tissues
    • Upright Olympus BX51 microscope: with colour camera for brightfield imaging
    • Leica MZ FLIII fluorescence stereomicroscope: for large field-of-view
    • Image analysis stations: three stations equipped with ImagePro Plus, Volocity, Metamorph, AutoQuant deconvolution, Adobe Illustrator,  Adobe Photoshop, ImageJ and Aperio Genie pattern recognition software
  • TGH (Max Bell, 1st floor, Room 1R402)
    • Two Olympus FluoView 1000 confocal microscopes: with SIM scanners for FRAP, spectral detectors and incubators for live cells
    • Two Yokogawa spinning disc confocal microscopes: real-time confocal imaging
    • Zeiss AxioObserver inverted microscope: with Apotome, incubator, and LED illumination for long-term multi-location time-lapse imaging
    • Total Internal Reflection Fluorescence (TIRF) microscope: for high-res imaging of biological surfaces
    • Laser Capture Microdissection (LCM) system
    • Image analysis station: equipped with Imaris, ImagePro Plus, Volocity, Metamorph, AutoQuant deconovolution, Slidebook, Adobe Illustrator, Adobe Photoshop
  • PM Cancer Centre (11th floor, ARC)
    • Xenogen IVIS Spectrum in vivo imaging systems: for quantitative, non-invasive bioluminescence and fluorescence imaging of living animals and cells, including spectral un-mixing and 3D tomography
Equipment
For a complete list of our equipment, please visit our website: http://www.aomf.ca/equipment.html.
Using the Facility
Users
  • Equipment and services available for UNH investigators and external users
  • Trained users book using online calendar, maximum 8 business hours/week/instrument

Training

  • Preliminary training by AOMF staff is mandatory, or ask about our full-service imaging
  • General courses offered on optical microscopy and image analysis
  • For a complete list of courses: http://www.aomf.ca/coursesmain.html

Pricing

Sample Preparation
  • Cell culture facilities are available at our TGH site. Please contact us for more information.
Contacts


James Jonkman, General manager
PMCRT 15-305
416-581-8593

Miria Bartolini, PMCRT site
PMCRT 15-301
416-581-8594

Judy Cathcart, PMCRT site
PMCRT 15-301
416-581-8594

Feng Xu, MBRC site
MBRC 4R410-R7
416-581-8612

Andrew Elia, PMCC site
PMCC, 7-305
416-946-4501 x5893

A multi-site facility (PMCRT, TGH and the PM) providing optical microscopy technologies for the observation of fixed cells, live cells, and tissues. The facility is led by Drs. Brian Wilson and Ming-Sound Tsao.
 

Services
 
  • Free consultations to determine which microscopes best suit your application
  • Comprehensive training and support on our various instruments
  • Full-service image capture and analysis (a great way to get started quickly)
  • High-resolution whole-slide scanning service for histology/pathology slides
  • Practical courses on microscopy, including one-on-one hands-on sessions
    (eg: Fundamentals of Microscopy) and larger workshops tailored to your group's applications
  • Software and training for image processing and quantification
Available Equipment
  • PMCRT (15th floor, Room 15-305)
    • Aperio Scanscope XT whole-slide scanner: digitizes entire stained histology slides at incredible resolution
    • New Zeiss LSM700 confocal microscope: optimized for DAPI/FITC/Cy3/Cy5 or equivalent
    • Zeiss LSM710 Two-Photon microscope (and fully-loaded confocal): upright microscope stand optimized for intravital imaging of mice and Second Harmonic Generation (SHG) microscopy
    • Zeiss AxioObserver: inverted microscope with incubator for time lapse live-cell imaging
    • Olympus BX50 motorized upright fluorescence microscope: for multi-colour fast tiling of whole slides
    • Zeiss AxioImager Z1 upright fluorescence microscope: has both monochrome and colour cameras
    • Renishaw Raman microscope: with inverted microscope stand for live-celll imaging, 3 lasers, and highspeed Raman mapping mode
    • CompuCyte iCys laser scanning cytometer: for multi-parameter analysis of cells and tissues
    • Upright Olympus BX51 microscope: with colour camera for brightfield imaging
    • Leica MZ FLIII fluorescence stereomicroscope: for large field-of-view
    • Image analysis stations: three stations equipped with ImagePro Plus, Volocity, Metamorph, AutoQuant deconvolution, Adobe Illustrator,  Adobe Photoshop, ImageJ and Aperio Genie pattern recognition software
  • TGH (Max Bell, 1st floor, Room 1R402)
    • Two Olympus FluoView 1000 confocal microscopes: with SIM scanners for FRAP, spectral detectors and incubators for live cells
    • Two Yokogawa spinning disc confocal microscopes: real-time confocal imaging
    • Zeiss AxioObserver inverted microscope: with Apotome, incubator, and LED illumination for long-term multi-location time-lapse imaging
    • Total Internal Reflection Fluorescence (TIRF) microscope: for high-res imaging of biological surfaces
    • Laser Capture Microdissection (LCM) system
    • Image analysis station: equipped with Imaris, ImagePro Plus, Volocity, Metamorph, AutoQuant deconovolution, Slidebook, Adobe Illustrator, Adobe Photoshop
  • PM Cancer Centre (11th floor, ARC)
    • Xenogen IVIS Spectrum in vivo imaging systems: for quantitative, non-invasive bioluminescence and fluorescence imaging of living animals and cells, including spectral un-mixing and 3D tomography
Equipment
For a complete list of our equipment, please visit our website: http://www.aomf.ca/equipment.html.
Using the Facility
Users
  • Equipment and services available for UNH investigators and external users
  • Trained users book using online calendar, maximum 8 business hours/week/instrument

Training

  • Preliminary training by AOMF staff is mandatory, or ask about our full-service imaging
  • General courses offered on optical microscopy and image analysis
  • For a complete list of courses: http://www.aomf.ca/coursesmain.html

Pricing

Sample Preparation
  • Cell culture facilities are available at our TGH site. Please contact us for more information.
Contacts


James Jonkman, General manager
PMCRT 15-305
416-581-8593

Miria Bartolini, PMCRT site
PMCRT 15-301
416-581-8594

Judy Cathcart, PMCRT site
PMCRT 15-301
416-581-8594

Feng Xu, MBRC site
MBRC 4R410-R7
416-581-8612

Andrew Elia, PMCC site
PMCC, 7-305
416-946-4501 x5893