Flow Cytometry at PMCRT and PGCRL (Sickkids-UHN Facility)

At Princess Margaret Cancer Research Tower (PMCRT) 2nd Floor, Rm 2-203/2-504 and the Peter Gilgan Centre for Research and Learning (PGCRL), 18th Floor, Rm 18-9430. Led by Dr. Cynthia Guidos and Dr. John Dick.

Services

Expert technical staff on site to operate and maintain the equipment, assist researchers in protocol selection, development and training.

What we offer:

  • Access to state-of-the-art analytical cytometers and high speed cell sorters (Note: sorting is performed by SickKids-UHN FCF operators)
  • CyTOF mass cytometry
  • Analytical mass cytometry (operator assisted)
  • Validated mass cytometry antibodies
  • Antibody metal labeling service
Available Equipment
  • Analytical Cytometers
    • Two BD FACSCanto II analytical cytometers (3-lasers: B, R, V, 8-colours)
    • BD LSRII analytical cytometer (4 lasers: B, R, V, UV, 14-colours)
    • BD LSRII analytical cytometer (4 lasers: B, R, V, UV, 16-colours)
    • BD LSRII analytical cytometer (4 lasers: B, G, R, V, 15-colours)
    • BD LSRII analytical cytometer (4 lasers: B, G, R, V, 18-colours)
    • BD LSRFortessa analytical cytometers (5 lasers: B, Y, R, V, UV, 14-colours)
    • DVS Sciences CyTOF 2 Mass Cytometer
    • ThermoFisher Attune analytical cytometer (2 lasers: B, R , 6-colours)
  • Cell sorters with a Bio-safety Cabinet
    • Beckman Coulter MoFlo XDP high-speed cell sorter (4 lasers: B, R, V, UV, 14-colours 4-way sorting)
    • Beckman Coulter MoFlo Astrios high-speed cell sorter (4 lasers: B, Y, R, V, 14-colours, 6-way sorting)
    • Two BD FACSAria II high-speed cell sorters (3 lasers: B, R, V, 11-colours, 4-way sorting)
    • Two BD FACSAria III high-speed cell sorters (4 lasers: B, R, Y, V, 17-colours, 4-way sorting)
    • BD InFlux high-speed cell sorter (3 lasers: B, R, V, 8-colours, 4-way sorting)
    • Sony SH800 high-speed cell sorter (3 lasers B, R, V , 6 colours-collinear lasers)
       
  • Magnetic Cell Separator
    • Two Miltenyi Biotec AutoMACS Pro magnetic cell separators
Using the Facility
  • Open to Investigators at UHN, SickKids, U of T, external academic and non-academic investigators
  • Please see website for specific instrument locations
  • First-come, first-serve priority system, with booking required

Training
To use any of the analytical cytometers or automated magnetic cell separators users must receive training from FCF staff.

Sample Preparation
  • Cell labelling must be performed in the investigator’s own laboratory before processing
  • Users must a gree to follow established SOPs and FCF policies and procedures.

Usage Arrangements
Contacts

SickKids-UHN Flow Cytometry Facility
Dr. Sherry Zhao
PMCRT Phone: 416-581-7643, Lab: 416-581-7654
PGCRL Phone: 416-813-7654 (ext. 309086), Lab: 416-813-7654 (ext. 309103)

At Princess Margaret Cancer Research Tower (PMCRT) 2nd Floor, Rm 2-203/2-504 and the Peter Gilgan Centre for Research and Learning (PGCRL), 18th Floor, Rm 18-9430. Led by Dr. Cynthia Guidos and Dr. John Dick.

Services

Expert technical staff on site to operate and maintain the equipment, assist researchers in protocol selection, development and training.

What we offer:

  • Access to state-of-the-art analytical cytometers and high speed cell sorters (Note: sorting is performed by SickKids-UHN FCF operators)
  • CyTOF mass cytometry
  • Analytical mass cytometry (operator assisted)
  • Validated mass cytometry antibodies
  • Antibody metal labeling service
Available Equipment
  • Analytical Cytometers
    • Two BD FACSCanto II analytical cytometers (3-lasers: B, R, V, 8-colours)
    • BD LSRII analytical cytometer (4 lasers: B, R, V, UV, 14-colours)
    • BD LSRII analytical cytometer (4 lasers: B, R, V, UV, 16-colours)
    • BD LSRII analytical cytometer (4 lasers: B, G, R, V, 15-colours)
    • BD LSRII analytical cytometer (4 lasers: B, G, R, V, 18-colours)
    • BD LSRFortessa analytical cytometers (5 lasers: B, Y, R, V, UV, 14-colours)
    • DVS Sciences CyTOF 2 Mass Cytometer
    • ThermoFisher Attune analytical cytometer (2 lasers: B, R , 6-colours)
  • Cell sorters with a Bio-safety Cabinet
    • Beckman Coulter MoFlo XDP high-speed cell sorter (4 lasers: B, R, V, UV, 14-colours 4-way sorting)
    • Beckman Coulter MoFlo Astrios high-speed cell sorter (4 lasers: B, Y, R, V, 14-colours, 6-way sorting)
    • Two BD FACSAria II high-speed cell sorters (3 lasers: B, R, V, 11-colours, 4-way sorting)
    • Two BD FACSAria III high-speed cell sorters (4 lasers: B, R, Y, V, 17-colours, 4-way sorting)
    • BD InFlux high-speed cell sorter (3 lasers: B, R, V, 8-colours, 4-way sorting)
    • Sony SH800 high-speed cell sorter (3 lasers B, R, V , 6 colours-collinear lasers)
       
  • Magnetic Cell Separator
    • Two Miltenyi Biotec AutoMACS Pro magnetic cell separators
Using the Facility
  • Open to Investigators at UHN, SickKids, U of T, external academic and non-academic investigators
  • Please see website for specific instrument locations
  • First-come, first-serve priority system, with booking required

Training
To use any of the analytical cytometers or automated magnetic cell separators users must receive training from FCF staff.

Sample Preparation
  • Cell labelling must be performed in the investigator’s own laboratory before processing
  • Users must a gree to follow established SOPs and FCF policies and procedures.

Usage Arrangements
Contacts

SickKids-UHN Flow Cytometry Facility
Dr. Sherry Zhao
PMCRT Phone: 416-581-7643, Lab: 416-581-7654
PGCRL Phone: 416-813-7654 (ext. 309086), Lab: 416-813-7654 (ext. 309103)