The Real-time PCR facility is a fee-for-service facility with sites at Princess Margaret (PM) Cancer Centre, Krembil Discovery Tower (KDT), and Princess Margaret Cancer Research Tower (PMCRT, formerly TMDT). The facilities are managed by Research Laboratory Services.

Strategic Research Initiatives Development
Available Equipment

Annoucement:

  • Sept. 28th 2017: We have 1 new Real Time PCR machine from Thermo for Krembil Community. Please see below for the location

  • Jun. 1st 2015: We have 4 more Real Time PCR machine from Roche for OCI Community. 3 are located at PM and 1 is located in PMCRT (formerly TMDT). Please see below for the locations

  • Anyone would like to book the new machine, please contact Wynne Yiu

Site

System

PM

Applied Biosystems Sequence Detection Systems 7900HT

  • 2 units available at PM (610 University Ave.), Rm 9-206/8-206

Roche Light Cycler 480 System

  • 1 unit available at PM (610 University Ave.), Rm 7-206

  • 2 units available at PM (610 University Ave.), Rm 9-314

KDT

Applied Biosystems Sequence Detection Systems 7900HT

  • 1 unit available at KDT, Rm 5KD443

Thermo QuantStudio 5 RT-PCR System

  • 1 unit available at KDT, Rm 5KD443

PMCRT
(formerly TMDT)

Applied Biosystems Sequence Detection Systems 7900HT

  • 1 unit available at PMCRT, Rm 13-601

Roche LightCycler 480 System

  • 1 unit available at PMCRT, Rm 13-601

 

 


 
Using the Facility
  • To use or book the instruments, you must be a registered user. Please contact Wynne Yiu for registration
  • Users can book the instrument up to 14 days in advance using the Online Calendar
  • Each time slot is 2.5 hours, which allows 2 runs and brief data analysis
  • Minimum 24 hours notification is required for any cancelations

Training
See the following documents before starting your RT-PCR experiments:

Techinical Support
Quick Links
Contacts

Account Registration (All sites):
Wynne Yiu, Research Analyst (416-581-7657)

Support:
Site: PM/PMCRT (formerly TMDT)
Malcolm Smith, Supervisor (416-946-4544)
Site: KDT
Frank Vidic, Research Facilities Coordinator (416-603-6462)

 
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Core Facilities

The Real-time PCR facility is a fee-for-service facility with sites at Princess Margaret (PM) Cancer Centre, Krembil Discovery Tower (KDT), and Princess Margaret Cancer Research Tower (PMCRT, formerly TMDT). The facilities are managed by Research Laboratory Services.

Available Equipment

Annoucement:

  • Sept. 28th 2017: We have 1 new Real Time PCR machine from Thermo for Krembil Community. Please see below for the location

  • Jun. 1st 2015: We have 4 more Real Time PCR machine from Roche for OCI Community. 3 are located at PM and 1 is located in PMCRT (formerly TMDT). Please see below for the locations

  • Anyone would like to book the new machine, please contact Wynne Yiu

Site

System

PM

Applied Biosystems Sequence Detection Systems 7900HT

  • 2 units available at PM (610 University Ave.), Rm 9-206/8-206

Roche Light Cycler 480 System

  • 1 unit available at PM (610 University Ave.), Rm 7-206

  • 2 units available at PM (610 University Ave.), Rm 9-314

KDT

Applied Biosystems Sequence Detection Systems 7900HT

  • 1 unit available at KDT, Rm 5KD443

Thermo QuantStudio 5 RT-PCR System

  • 1 unit available at KDT, Rm 5KD443

PMCRT
(formerly TMDT)

Applied Biosystems Sequence Detection Systems 7900HT

  • 1 unit available at PMCRT, Rm 13-601

Roche LightCycler 480 System

  • 1 unit available at PMCRT, Rm 13-601

 

 


 
Using the Facility
  • To use or book the instruments, you must be a registered user. Please contact Wynne Yiu for registration
  • Users can book the instrument up to 14 days in advance using the Online Calendar
  • Each time slot is 2.5 hours, which allows 2 runs and brief data analysis
  • Minimum 24 hours notification is required for any cancelations

Training
See the following documents before starting your RT-PCR experiments:

Techinical Support
Quick Links
Contacts

Account Registration (All sites):
Wynne Yiu, Research Analyst (416-581-7657)

Support:
Site: PM/PMCRT (formerly TMDT)
Malcolm Smith, Supervisor (416-946-4544)
Site: KDT
Frank Vidic, Research Facilities Coordinator (416-603-6462)

 
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015
Documents and Forms
Form Name Purpose Version Date
Roche LightCycler 480 Absolute Quantification Quick Reference Card Roche LightCycler 480 Jul 15, 2015
Roche LightCycler 480 Chemistry Protocols Quick Reference Card Roche LightCycler 480 Chemistry Protocols Quick Reference Card Jul 15, 2015
Roche LightCycler 480 Overview Roche LightCycler 480 Overview Jul 15, 2015
Roche LightCycler 480 Relative Quantification Quick Reference Guide Roche LightCycler 480 Relative Quantification Quick Reference Guide Jul 15, 2015