Suzanne Kamel-Reid, PhD

Cancer Clinical Research Unit (CCRU), Princess Margaret Cancer Centre
Keywords: leukemia, head and neck cancers, comparative genomic hybridization (CGH), spectral karyotyping, lovastatin, acute promyelocytic leukemia (APL)
Identifying biomarkers of cancer initiation, progression and recurrence

Work in my laboratory focuses on the application of high throughput technologies (e.g., gene expression microarrays, tissue arrays, ChIP-on-chip, microRNA arrays, protein arrays) to enhance our understanding of cancer biology. Specifically, we are interested in identifying biomarkers of cancer initiation, progression and recurrence in two types of human cancer: head and neck squamous cell carcinoma (HNSCC) and acute promyelocytic leukemia (APL).
  • Head and neck squamous cell carcinoma
    In this arm of my research laboratory, we are specifically interested in the etiology of oral cancers (OSCCs). The molecular genetic changes involved in oral cancer development are poorly understood. Our work focuses on three major questions:
    1. What are the gene(s) involved in recurrence of oral cancer? Approximately 50% of OSCCs recur after surgery. Our current approaches involve analyzing OSCC tumour samples, as well as samples taken from the regions immediately surrounding the tumours at time of surgery (''surgical resection margins'') in order to identify genes deregulated in the tumour and in the surrounding margin(s), which may be predictive of tumour recurrence.
    2. What are the steps involved in the development of oral cancer? A significant fraction of OSCCs arise from precursor lesions called leukoplakias. In these studies, we are interested in profiling the genetic changes—specifically, changes in microRNA expression—in leukoplakias and comparing them to genetic changes found in tumours.
    3. What are the underlying genetic differences between OSCCs in young patients (<45 yrs of age) and older patients (>45 yrs of age)? OSCCs are strongly associated with the risk factors of alcohol consumption and smoking tobacco. However, younger patients who do not exhibit exposure to either of these two risk factors are still diagnosed with OSCC. Our previous work has demonstrated that genetic differences exist between young and older patients. Specifically, we have observed defective DNA mismatch repair and differential gene expression in young patients compared to older patients. This line of study is aimed at further understanding the genetic differences between young and older patients, and at understanding the role of defective DNA repair in OSCCs.
  • Acute promyelocytic leukemia
    Leukemias are often associated with chromosomal translocations that give rise to fusion proteins which may deregulate cellular signaling or transcription. Our group cloned and characterized two variant fusion proteins involved in acute promyelocytic leukemia (APL), NPM-RAR-alpha and NuMA-RAR-alpha, which are both aberrant transcription factors; we are especially interested in understanding the roles of these fusion proteins in the cell. Specifically, we use cell lines, mouse models and human patient samples in order to understand leukemia biology. The questions we are most interested in include:
    1. What are the cell biology effects of the APL fusion proteins? While most studies have focused on the APL fusions as transcription factors, more recent evidence has suggested that these proteins behave distinctly and have effects through protein-protein interactions on other signaling pathways within the leukemic cell. We are applying the same concept as above, and hypothesizing that there may be a common set of interactions and/or deregulated pathways shared by all APL fusion proteins, and are characterizing the protein-protein interactions of the fusions in order to find this out.
    2. What are the necessary secondary events in leukemia development? Previous studies have indicated that the APL fusions are necessary, but insufficient, for the development of leukemia in mice. This suggests that additional genetic ''events'' may cooperate with the fusions in leukemia. We are addressing this question through the above studies, and also through genetic studies of the hCG-NuMA-RAR-alpha mouse model that we previously developed and characterized.
    What are the common downstream genetic targets of the APL fusion proteins? APL is associated with seven known fusion proteins, all of which involve the RAR-alpha transcription factor. Yet, all fusion proteins give rise to the same disease. We hypothesize that this is because all fusion proteins have a common set of direct transcriptional targets, and are utilizing gene expression arrays and ChIP-on-chip technology to address this issue.
Distinct patterns of clonal evolution in patients with concurrent myelo- and lymphoproliferative neoplasms.
Blood. 2018 Sep 24;:
Kennedy JA, Medeiros JJF, Dobson SM, Arruda A, Sukhai MA, Stockley T, Tierens A, Minden MD, Kamel-Reid S, Dick JE, Gupta V
Genomic classifier for guiding treatment of intermediate-risk prostate cancers to dose-escalated image-guided radiotherapy without hormone therapy.
Int J Radiat Oncol Biol Phys. 2018 Aug 28;:
Berlin A, Murgic J, Hosni A, Pintilie M, Salcedo A, Fraser M, Kamel-Reid S, Zhang J, Wang Q, Ch'ng C, Deheshi S, Davicioni E, van der Kwast T, Boutros PC, Bristow RG, Chua MLK
Additional germline findings from a tumor profiling program.
BMC Med Genomics. 2018 Aug 09;11(1):65
Stjepanovic N, Stockley TL, Bedard PL, McCuaig JM, Aronson M, Holter S, Semotiuk K, Leighl NB, Jang R, Krzyzanowska MK, Oza AM, Gupta A, Elser C, Ahmed L, Wang L, Kamel-Reid S, Siu LL, Kim RH
The prognostic effect of single and multiple cancer-related somatic mutations in resected non-small-cell lung cancer.
Lung Cancer. 2018 Sep;123:22-29
Jao K, Tomasini P, Kamel-Reid S, Korpanty GJ, Mascaux C, Sakashita S, Labbé C, Leighl NB, Liu G, Feld R, Bradbury PA, Hwang DM, Pintilie M, Tsao MS, Shepherd FA
Lung cancer in never smokers from the Princess Margaret Cancer Centre.
Oncotarget. 2018 Apr 27;9(32):22559-22570
Korpanty GJ, Kamel-Reid S, Pintilie M, Hwang DM, Zer A, Liu G, Leighl NB, Feld R, Siu LL, Bedard PL, Tsao MS, Shepherd FA
Evidence-based best practices for EGFR T790M testing in lung cancer in Canada.
Curr Oncol. 2018 Apr;25(2):163-169
Stockley T, Souza CA, Cheema PK, Melosky B, Kamel-Reid S, Tsao MS, Spatz A, Karsan A
Translation of knowledge to practice - Improving awareness in NSCLC molecular testing.
J Thorac Oncol. 2018 Mar 10;:
Zer A, Cutz JC, Sekhon H, Hwang DM, Sit C, Maganti M, Sung M, Binnie M, Brade A, Chung TB, Kamel-Reid S, Narinder P, Tsao MS, Waddell T, da Cunha Santos G, Patel M, Carter RF, Leighl NB
AML refractory to primary induction with Ida-FLAG has a poor clinical outcome.
Leuk Res. 2018 Feb 20;68:22-28
Kavanagh S, Heath E, Hurren R, Gronda M, Barghout SH, Liyanage SU, Siriwardena TP, Claudio J, Zhang T, Sukhai M, Stockley TL, Kamel-Reid S, Rostom A, Lutynski A, Khalaf D, Rydlewski A, Chan SM, Gupta V, Maze D, Sibai H, Schuh AC, Yee K, Minden MD,...
Standardizing biomarker testing for Canadian patients with advanced lung cancer.
Curr Oncol. 2018 Feb;25(1):73-82
Melosky B, Blais N, Cheema P, Couture C, Juergens R, Kamel-Reid S, Tsao MS, Wheatley-Price P, Xu Z, Ionescu DN
Prognostic Significance of Human Papilloma Virus and p16 Expression in Patients with Vulvar Squamous Cell Carcinoma who Received Radiotherapy.
Clin Oncol (R Coll Radiol). 2018 Feb 12;:
Yap ML, Allo G, Cuartero J, Pintilie M, Kamel-Reid S, Murphy J, Mackay H, Clarke B, Fyles A, Milosevic M

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Cancer Clinical Research Unit (CCRU), Princess Margaret Cancer Centre

Professor, Departments of Medical Biophysics and Laboratory Medicine and Pathobiology, University of Toronto
Head, Laboratory Genetics, University Health Network
Director, Molecular Diagnostics, University Health Network
Member, Ontario Genetics Secretariat Executive
Co-chair, Molecular Oncology Advisory Committee, Cancer Care Ontario​