The Wheeler lab employs a systems-physiology approach to research using innovative high-throughput techniques, incorporating mass-spectrometry and the membrane yeast two-hybrid system. This approach enables the Wheeler lab to perform powerful screens capable of revealing novel targets at an unprecedented rate. These screens are complemented by primary research that utilizes primary cells, cell lines and mouse models to ensure the results are biologically relevant. The lab focuses on two exciting research streams:
Michael B Wheeler
- Assessing the Diabetic Effect on Metabolism in Humans using MetabolomicsThe Wheeler lab is exploring the use of metabolomics as a tool to directly assess the diabetic effect on metabolism in humans. Metabolomics is a recent technological advance that characterizes metabolites, the final compounds of chemical reactions and those most relevant to metabolic diseases such as diabetes. This approach forms the basis of multiple predictive- and causative-oriented investigations in our lab for gestational and type 2 diabetes.
- Identification of GLP-1R Interactors as Potential Drug Targets for Type II DiabetesUsing high-throughput technologies, incorporating mass spectrometry and MYTH screening, the Wheeler lab has identified a large number proteins that interact with the glucagon like peptide-1 receptor (GLP-1R) in a variety of tissues, including mouse and human islets. Following the identification and verification of GLP-1R interactors, members of the Wheeler lab characterize potential functional changes to glucose stimulated insulin secretion, GLP-1 binding, and proliferation and survival. Ultimately, candidate GLP-1R interactors that elicit significant functional changes are selected for further mechanistic studies. Identifying and characterizing GLP-1R interactors may reveal potential drug targets that can be utilized to improve the lives of those living with type 2 diabetes.
J Biomed Opt. 2018 Feb;23(2):1-6
Elevated Medium Chain-Acylcarnitines are Associated with Gestational Diabetes, and Early Progression to Type-2 Diabetes, and Induce Pancreatic β-Cell Dysfunction.
Diabetes. 2018 Feb 07;:
Holo-lipocalin-2-derived siderophores increase mitochondrial ROS and impair oxidative phosphorylation in rat cardiomyocytes.
Proc Natl Acad Sci U S A. 2018 Jan 29;:
Cell Tissue Res. 2018 Jan 09;:
CMPF, a Metabolite Formed Upon Prescription Omega-3-Acid Ethyl Ester Supplementation, Prevents and Reverses Steatosis.
EBioMedicine. 2017 Dec 19;:
Relationship between follicular dynamics and oocyte maturation during in vitro culture as a non-invasive sign of caprine oocyte meiotic competence.
Theriogenology. 2017 Nov 04;107:95-103
Nat Commun. 2017 Aug 08;8(1):210
Diabetologia. 2017 Jul 20;:
Uncoupling protein 2 regulates daily rhythms of insulin secretion capacity in MIN6 cells and isolated islets from male mice.
Mol Metab. 2017 Jul;6(7):760-769
Senior Scientist, Toronto General Hospital Research Institute (TGHRI)
Professor, University of Toronto, Department of Physiology, Faculty of Medicine