The Wheeler lab employs a systems-physiology approach to research using innovative high-throughput techniques, incorporating mass-spectrometry and the membrane yeast two-hybrid system. This approach enables the Wheeler lab to perform powerful screens capable of revealing novel targets at an unprecedented rate. These screens are complemented by primary research that utilizes primary cells, cell lines and mouse models to ensure the results are biologically relevant. The lab focuses on two exciting research streams:
- Assessing the Diabetic Effect on Metabolism in Humans using MetabolomicsThe Wheeler lab is exploring the use of metabolomics as a tool to directly assess the diabetic effect on metabolism in humans. Metabolomics is a recent technological advance that characterizes metabolites, the final compounds of chemical reactions and those most relevant to metabolic diseases such as diabetes. This approach forms the basis of multiple predictive- and causative-oriented investigations in our lab for gestational and type 2 diabetes.
- Identification of GLP-1R Interactors as Potential Drug Targets for Type II DiabetesUsing high-throughput technologies, incorporating mass spectrometry and MYTH screening, the Wheeler lab has identified a large number proteins that interact with the glucagon like peptide-1 receptor (GLP-1R) in a variety of tissues, including mouse and human islets. Following the identification and verification of GLP-1R interactors, members of the Wheeler lab characterize potential functional changes to glucose stimulated insulin secretion, GLP-1 binding, and proliferation and survival. Ultimately, candidate GLP-1R interactors that elicit significant functional changes are selected for further mechanistic studies. Identifying and characterizing GLP-1R interactors may reveal potential drug targets that can be utilized to improve the lives of those living with type 2 diabetes.
Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells.
J Mech Behav Biomed Mater. 2019 Mar 22;95:21-33
Pharmacologic or genetic activation of SIRT1 attenuates the fat-induced decrease in beta-cell function in vivo.
Nutr Diabetes. 2019 Mar 19;9(1):11
Non-invasive nuclear magnetic resonance analysis of male and female embryo metabolites during in vitro embryo culture.
Metabolomics. 2018 Aug 25;14(9):113
Correction to: The discovery of novel predictive biomarkers and early-stage pathophysiology for the transition from gestational diabetes to type 2 diabetes.
Diabetologia. 2019 Feb 08;:
The discovery of novel predictive biomarkers and early-stage pathophysiology for the transition from gestational diabetes to type 2 diabetes.
Diabetologia. 2019 Jan 15;:
SLIM microscopy allows for visualization of DNA-containing liposomes designed for sperm-mediated gene transfer in cattle.
Mol Biol Rep. 2018 Dec 11;:
GABA promotes β-cell proliferation, but does not overcome impaired glucose homeostasis associated with diet-induced obesity.
FASEB J. 2018 Dec 03;:fj201801397R
Cilostamide affects in a concentration and exposure time-dependent manner the viability and the kinetics of in vitro maturation of caprine and bovine oocytes.
Res Vet Sci. 2018 Nov 12;122:22-28
3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF) Prevents HFD Induced Insulin Resistance via the Maintenance of Hepatic Lipid Homeostasis.
Diabetes Obes Metab. 2018 Jul 30;:
J Mol Endocrinol. 2018 Aug;61(2):69-77
Senior Scientist, Toronto General Hospital Research Institute (TGHRI)
Professor, University of Toronto, Department of Physiology, Faculty of Medicine