Gregory P Downey

Gregory P Downey, BSc, MD, FRCPC

My research examines the intracellular signaling events regulating leukocyte activation, an important process that occurs in diseases characterized by inflammatory injury such as acute lung injury (ARDS), sepsis, ischemia reperfusion, cystic fibrosis and asthma.

Our laboratory uses state-of-the-art techniques in cell and molecular biology including single cell microfluoresence imaging, microinjection, cloning and sequencing of genomic DNA, PCR, site-directed mutagenesis, fusion protein expression, mammalian cell transfection with dominant negative proteins and the study of knockout mice.

Ongoing projects include:
  • The characterization of tyrosine phosphatases expressed in leukocytes including SHP-1, SHP-2, and MEG-2. We are studying mice lacking these protein tyrosine phosphatases. A wide range of effector cell functions are being studied in these mice including phagocytosis, oxidative burst, tyrosine phosphorylation and other signaling pathways.
  • The role of adhesion molecules in cell signaling. This involves the study of the signaling pathways activated by beta-2 integrins and L-selectin leading to leukocyte activation. Signaling pathways involving MAP kinases and Akt are being investigated.
  • Interaction of neutrophils with bronchial epithelial cells with specific reference to the expression of the cystic fibrosis transmembrane regulatory (CFTR) protein on these interactions. This project involves the use of single cell microfluorescence techniques investigating the interaction of isolated neutrophils with bronchial epithelial cells in culture.
Am J Respir Crit Care Med. 2017 Sep 20;:
Kumar SR, Paudel S, Ghimire L, Bergeron S, Cai S, Zemans RL, Downey GP, Jeyaseelan S
Ann Am Thorac Soc. 2016 Oct;13(10):1671-1673
Downey GP, Aschner Y
Am J Respir Crit Care Med. 2016 Sep 15;194(6):748-61
McCormack FX, Gupta N, Finlay GR, Young LR, Taveira-DaSilva AM, Glasgow CG, Steagall WK, Johnson SR, Sahn SA, Ryu JH, Strange C, Seyama K, Sullivan EJ, Kotloff RM, Downey GP, Chapman JT, Han MK, D'Armiento JM, Inoue Y, Henske EP, Bissler JJ, Colby TV,...
Compr Physiol. 2016;6(2):945-73
Schmidt EP, Kuebler WM, Lee WL, Downey GP
Br J Gen Pract. 2015 Sep;65(638):474
Black A, Singh A, Downey GP
Ann Am Thorac Soc. 2015 Mar;12 Suppl 1:S1-2
Riches DW, Burnham EL, Moss M, Downey GP, Schmidt EP
Chest. 2014 Oct 1;146(4):1081-91
Aschner Y, Zemans RL, Yamashita CM, Downey GP