Anthony Gramolini

Anthony Gramolini, PhD

Our laboratory is interested in the cellular mechanisms that result in the proper formation of a specialized membrane domain within muscle, termed the sarcoplasmic reticulum. The sarcoplasmic reticulum is an internal membrane system in muscle that regulates calcium uptake and release in excitation-contraction. We employ a range of current molecular and cellular approaches, as well as advanced proteomic and genomic methods, to investigate the physiology and biochemistry of several key Ca2+ regulatory proteins in cardiac and skeletal muscle.

In particular, we are interested in:

  1. The factors that regulate Ca2+ release and uptake in the sarcoplasmic reticulum
  2. The cellular consequences of dysregulation of Ca2+ uptake
For these experiments, we apply conventional biochemical and molecular biology assays together with digital confocal imaging. We are also applying advanced proteomic detection methods as well as protein-protein interaction methods to decipher critical events and functions in cardiac muscle. Model systems include established cell lines, primary cell cultures and transgenic animals. These techniques serve as valuable experimental tools to investigate human mutations in calcium regulatory proteins, cellular protein sorting and Ca2+ cycling.

Related Links

Am J Physiol Cell Physiol. 2017 Nov 22;:ajpcell.00141.2017
Lee SH, Hadipour-Lakmehsari S, Miyake T, Gramolini AO
J Am Heart Assoc. 2017 Feb 07;6(2):
Mofid A, Newman NS, Lee PJ, Abbasi C, Matkar PN, Rudenko D, Kuliszewski MA, Chen HH, Afrasiabi K, Tsoporis JN, Gramolini AO, Connelly KA, Parker TG, Leong-Poi H
Proc Natl Acad Sci U S A. 2016 Oct 14;:
Kuzmanov U, Guo H, Buchsbaum D, Cosme J, Abbasi C, Isserlin R, Sharma P, Gramolini AO, Emili A
Nat Commun. 2016;7:11906
Kim Y, Jeon J, Mejia S, Yao CQ, Ignatchenko V, Nyalwidhe JO, Gramolini AO, Lance RS, Troyer DA, Drake RR, Boutros PC, Semmes OJ, Kislinger T
Nat Immunol. 2015 Dec 7;
Ensan S, Li A, Besla R, Degousee N, Cosme J, Roufaiel M, Shikatani EA, El-Maklizi M, Williams JW, Robins L, Li C, Lewis B, Yun TJ, Lee JS, Wieghofer P, Khattar R, Farrokhi K, Byrne J, Ouzounian M, Zavitz CC, Levy GA, Bauer CM, Libby P, Husain M,...
Nat Commun. 2015;6:8391
Sharma P, Abbasi C, Lazic S, Teng AC, Wang D, Dubois N, Ignatchenko V, Wong V, Liu J, Araki T, Tiburcy M, Ackerley C, Zimmermann WH, Hamilton R, Sun Y, Liu PP, Keller G, Stagljar I, Scott IC, Kislinger T, Gramolini AO
PLoS One. 2015;10(8):e0135755
Spillane DR, Wang DY, Newbigging S, Wang Y, Shi CX, Cho HR, Shimizu H, Gramolini A, Liu M, Wen XY
Proc Natl Acad Sci U S A. 2015 May 26;
Teng AC, Miyake T, Yokoe S, Zhang L, Rezende LM, Sharma P, MacLennan DH, Liu PP, Gramolini AO



Associate Professor, Department of Physiology, University of Toronto
Tier 2 Canada Research Char in Cardiovascular Proteomics and Molecular Therapeutics
Member, Richard Lewar / Heart & Stroke Centre of Excellence