Anthony Gramolini, PhD

Our laboratory is interested in the cellular mechanisms that result in the proper formation of a specialized membrane domain within muscle, termed the sarcoplasmic reticulum. The sarcoplasmic reticulum is an internal membrane system in muscle that regulates calcium uptake and release in excitation-contraction. We employ a range of current molecular and cellular approaches, as well as advanced proteomic and genomic methods, to investigate the physiology and biochemistry of several key Ca2+ regulatory proteins in cardiac and skeletal muscle.

In particular, we are interested in:

  1. The factors that regulate Ca2+ release and uptake in the sarcoplasmic reticulum
  2. The cellular consequences of dysregulation of Ca2+ uptake
For these experiments, we apply conventional biochemical and molecular biology assays together with digital confocal imaging. We are also applying advanced proteomic detection methods as well as protein-protein interaction methods to decipher critical events and functions in cardiac muscle. Model systems include established cell lines, primary cell cultures and transgenic animals. These techniques serve as valuable experimental tools to investigate human mutations in calcium regulatory proteins, cellular protein sorting and Ca2+ cycling.

Related Links

For a list of Dr. Gramolini's publications, please visit PubMed or Scopus.

Associate Professor, Department of Physiology, University of Toronto
Tier 2 Canada Research Char in Cardiovascular Proteomics and Molecular Therapeutics
Member, Richard Lewar / Heart & Stroke Centre of Excellence