maximum number of expected oligo probes per sequence/cluster.
GUI default = 16
max: 32
min: 1
type: integer

minimum oligo length
Default = 20 - has to be equal or smaller than the maximum oligo length
max: 70
min: 20
type: integer

maximum oligo length
Default = 70 has to be equal or larger than minimum oligo length
max: 70
min: 20
type: integer

minimum oligo melting temperature
Default = 55 - has to be equal or smaller than maximum temperature
max: 95
min: 35
type: integer

maximum oligo melting temperature
Default = 95 - has to be equal or larger than minimum temp
max: 95
min: 35
type: integer

Minimum and maximum oligo melting temperature can be replaced by

temperature range
Default = 5
type: integer

minimum oligo GC content
Default = 35
max: 50
min: 30
type: integer

maximum oligo GC content
Default = 65
max: 70
min: 50
type: integer

seed pattern - check here for more help
Default = #@#__##__#_##@#
use # for match
use @ for match or transition
use , for separate pattern
maximum number of patterns is 32
type: string

total DNA concentration (Molar)
Default = 0.00001
max: 1000
min: 0.00001
type: float

temperature used by hybrid-min for free energy calculation
Default = 65
max: 95
min: 35
type: integer

concentration of [Na+] (Molar)
Default = 1
max: 1000
min: 0.00001
type: float

concentration of [Mg+] (Molar)
Default = 0
max: 1000
min: 0.00001
type: float