Wright Cell Imaging Facility

 

 

 

Upright Confocal   Upright Widefield   Live Cell Inverted    Microdissection   Stereology   Offline Analysis

Post-acquisition Offline Workstation

This high-spec PC has a 3.2 GHz CPU, 2 Gb RAM and a GeForce 6800 Ultra graphics card. It has various image processing, analysis and presentation software packages installed. The facility can provide support and training for all these software packages.

Image Processing and Analysis
The main software used for processing and analysis is the WCIF ImageJ custom installation (Click here). Images can be processed with ImageJ before being imported in to CorelDRAW for preparation of publication-quality figures. Data from ImageJ can be exported directly to Excel for further analysis.

For widefield deconvolution we have Zeiss' Axiovision 4.2 which will perform deconvolution using a theoretical PSF. We also have AutoDeblur GOLD which will perform Blind deconvolution on widefield or confocal data.

For 3D reconstruction and analysis we use ImageJ running VolumeJ and also Bitplane's Imaris Suite.

A copy of Zeiss LSM510 3.2 is installed for offline analysis of LSM Lambda-stacks.

Image Presentation
For preparation of figures we have CorelDRAW software. This allows generation of figures containing both vector (e.g. line-graphs) and bitmap image types.

For animated presentations we have Macromedia's FLASH MX 2004. These animations can be embedded in to PowerPoint presentations or published online.

08/02/2006