|
 The microscope
is also equipped for 'Deconvolution'. Microscope images have an
intrinsic, largely predictable ‘blur’ due to the limited aperture of
the microscope objective lens. ‘Deconvolution’ is the name given to
a suite of techniques designed to correct this blur and improve the
original image. A number of algorithms are available, trading speed
of processing for final image quality. Deconvolution is considered
to be more 'photon-efficient' than confocal microscopes, although
lengthy post-acquisition processing is required.
This upright
microscope is equipped with a Zeiss AxioCam high resolution,
digital camera and Axiovision software providing
deconvolution software that uses a theoretical point-spread
function.
GFAP-positive (green) astrocyte from adult mouse hippocampus.
Sample provided by R. Logan (TWRI). Scale-bar 5 µm. Images are median
slices from z-series. Insets are magnified regions of the
cell-body. Panels are deconvolved with the following
algorithms (clockwise from top-left)) original data (no
deconvolution), nearest neighbour, inverse filter,
constrained iterative. |
