HeLa cells growing at 37°C in a sealed chamber with normal experimental buffer supplemented with 10 µM staurosporine to induce apoptosis; 2 µl/ml Annexin V-AlexaFluor 488 (green) to indicate phoshotydylserine flipping; and 2 µM propidium iodide (red) to reveal loss of membrane integrity. Images were acquired with a conventional widefield, fluorescence imaging system equipped with time-lapse functionality (Perkin Elmer) using a ×20 phase objective.
Images were acquired every 6 minutes. (T. Collins, Babraham Institute, UK).

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