Available Equipment
- Spinning disk confocal microscope for tracking nuclear DNA repair complexes
- PCR and qRT-PCR apparatuses
- DNA, RNA and protein analyses apparatuses
- Quantitative fluorescent COMET assay for measuring DNA damage
- Level 2 tissue culture facility
- Radioisotope laboratory for in vitro and metabolic labelling
- Prostate and bladder cancer xenografts endpoints for preclinical testing of novel agents
- Microenvironmental chambers to assess the effects of hypoxia on cellular models
- MesoScale Discovery Sector Imager 2400 for multiplex immunoassays for cytokine and proteomic studies during cancer therapy
Unique Capacities
Core I includes state-of-the-art microscopes for high-resolution longitudinal imaging of molecular processes within cells. It also includes equipment for gene and protein expression studies in patient tissues.
The proximity of Core I to the other Cores allows STTARR to more quickly assess the effectiveness of preclinical and clinical molecular agents. This helps researchers to quickly identify what Phase I agents can be used with radiotherapy.
Specialized facilities contained within this Core include:
The proximity of Core I to the other Cores allows STTARR to more quickly assess the effectiveness of preclinical and clinical molecular agents. This helps researchers to quickly identify what Phase I agents can be used with radiotherapy.
Specialized facilities contained within this Core include:
- Access to serum and tissue proteomic testing
- Preclinical and clinical tissue microarrays for correlative science studies
- Preclinical tumour and normal tissue assays for assessing efficacy and toxicity of novel cancer agents
- Immunohistochemical staining for radiation response pathways
- Microenvironmental facilities to measure radiation responses
- Specialized microscopy and image analysis facilities for live cell imaging studies
- DNA repair assays
Using the Facility
- Booking time on the microscopes is available to academic and external users
- The availability of the equipment for collaborative research may vary according to the nature and scope of ongoing projects
Usage Arrangements
- Collaborative and/or user fee; contact STTARR for costing estimates
- STTARR pricing structure can be found here: http://www.sttarr.ca/pricingstructure.pdf
Contacts
Dr. Gaetano Zafarana
Core I Manager
416-581-7760
Princess Margaret Cancer Research Tower
Room 7-201, 101 College Street
Toronto, Ontario, M5G 1L7
http://www.sttarr.com/contact
Core I Manager
416-581-7760
Princess Margaret Cancer Research Tower
Room 7-201, 101 College Street
Toronto, Ontario, M5G 1L7
http://www.sttarr.com/contact
The Cellular Core of STTARR supports DNA repair assays coupled to genomic and proteomic testing for the prediction of radiation response and toxicity. In addition to our support given to both basic and clinical studies relating to genetic instability and cancer risk, our infrastructure also provides a thriving scientific environment ideal for R&D and new drug feasibility studies as service or as laboratory space provided to pharmaceutical and product development companies.